In vitro cellular level - PharmaCore Labs (PCL) Co., Ltd

SERVICE

KEYWORD

CONTACT US

Main Office, PCL

Address: Haimen Biotech and Pharmaceutical Park, Riverside New District, Haimen, Jiangsu Province, China

Tel:+86 0513 68015200

Email:infor@pharmacorelab.com
Website: www.pharmacorelab.com


Branch Office, PCL

Address: Block 6D, 780 Cailun Road, China (Shanghai) pilot free trade zone. infor@pharmacorelab.com

In vitro cellular level

Your current location: Home > SERVICE > Preclinical cardiac safety > In vitro cellular level

Primary cardiac myocytes in guinea pig or rabbit - K channel

Time2020-04-20 15:24:32 Hits713


Pharmacore Labs currently provide manual patch clamp assay using acutely isolated animal cardiomyocytes to test drug's inhibition or potentiating effect on Ik1 mainly contributed by Kir2.1 channels.

In vitro electrophysiology IK1 assay in primary cardiomyocytes:

Assay platform/method

Conventional patch clamp

Cells used in the assay

Acutely isolated cardiomyocytes from guinea pigs, or rabbits

Measured parameters

Whole cell current of Ik1

Test concentrations

4 doses for IC50

Data point repeats

N ≥ 3 for each concentration

Note: 1) Positive control: 20 μM BaCl2 is used in the assay to ensure cell quality and normal response of Ik1 channels.


Ik1 current in guinea pig cardiomyocyte and blockade effect of 20 μM BaCl2

Copyright © PharmaCore Labs (PCL) Co., Ltd All rights reserved 备案号:苏ICP备15051795号 欢迎来电咨询! 技术支持:裕福科技