Main Office, PCL
Address: Haimen Biotech and Pharmaceutical Park, Riverside New District, Haimen, Jiangsu Province, China
Tel：+86 0513 68015200
Email:infor@pharmacorelab.com
Website: www.pharmacorelab.com
Branch Office, PCL
Address: Block 6D, 780 Cailun Road, China (Shanghai) pilot free trade zone. infor@pharmacorelab.com
Pharmacore Labs currently provide manual patch clamp assay using acutely isolated animal cardiomyocytes to test drug's inhibition or potentiating effect on Ik1 mainly contributed by Kir2.1 channels.
In vitro electrophysiology IK1 assay in primary cardiomyocytes:
|
Assay platform/method |
Conventional patch clamp |
|
Cells used in the assay |
Acutely isolated cardiomyocytes from guinea pigs, or rabbits |
|
Measured parameters |
Whole cell current of Ik1 |
|
Test concentrations |
4 doses for IC50 |
|
Data point repeats |
N ≥ 3 for each concentration |
Note: 1) Positive control: 20 μM BaCl2 is used in the assay to ensure cell quality and normal response of Ik1 channels.
Ik1 current in guinea pig cardiomyocyte and blockade effect of 20 μM BaCl2
