In vitro cellular level - PharmaCore Labs (PCL) Co., Ltd




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In vitro cellular level

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Primary cardiac myocytes in guinea pig or rabbit - K channel

Time2020-04-20 15:24:32 Hits510

Pharmacore Labs currently provide manual patch clamp assay using acutely isolated animal cardiomyocytes to test drug's inhibition or potentiating effect on Ik1 mainly contributed by Kir2.1 channels.

In vitro electrophysiology IK1 assay in primary cardiomyocytes:

Assay platform/method

Conventional patch clamp

Cells used in the assay

Acutely isolated cardiomyocytes from guinea pigs, or rabbits

Measured parameters

Whole cell current of Ik1

Test concentrations

4 doses for IC50

Data point repeats

N ≥ 3 for each concentration

Note: 1) Positive control: 20 μM BaCl2 is used in the assay to ensure cell quality and normal response of Ik1 channels.

Ik1 current in guinea pig cardiomyocyte and blockade effect of 20 μM BaCl2

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