Main Office, PCL
Address: Haimen Biotech and Pharmaceutical Park, Riverside New District, Haimen, Jiangsu Province, China
Tel：+86 0513 68015200
Email:infor@pharmacorelab.com
Website: www.pharmacorelab.com
Branch Office, PCL
Address: Block 6D, 780 Cailun Road, China (Shanghai) pilot free trade zone. infor@pharmacorelab.com
CiPA program requires drug's effect on cardiac ion channels and action potential to be verified in human ventricular iPS (induced pluropotent stem cell)-derived myocytes.
In vitro electrophysiology assay in human iPS-derived ventricular myocytes:
|
Assay platform/method |
Conventional patch clamp |
|
Cells used in the assay |
Human iPS-derived ventricular myocytes( IPSC) |
|
Measured parameters |
Action potential (APA, APD30/60/90) |
|
Test concentrations |
3 doses, 3 stimulation frequency (0.5 Hz, 1 Hz, 1.7 Hz) |
|
Data point repeats |
N = 3 for each concentration |
Note: 1) Positive control: 0.3 μM Haloperidol is used in the assay to ensure cell quality and normal response of sodium channels.
2) If requested, 1 or 2 stimulation frequency can be selected.
 |
 |
|
Effect of haloperidol on IPS-derived ventricular myocytes |
Effect of terfenadine on IPS-derived ventricular myocytes |
