Main Office, PCL
Address: Haimen Biotech and Pharmaceutical Park, Riverside New District, Haimen, Jiangsu Province, China
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Email:infor@pharmacorelab.com
Website: www.pharmacorelab.com
Branch Office, PCL
Address: Block 6D, 780 Cailun Road, China (Shanghai) pilot free trade zone. infor@pharmacorelab.com
In vitro electrophysiology ICa assay in primary cardiomyocytes:
|
Assay platform/method |
Conventional patch clamp |
|
Cells used in the assay |
Acutely isolated cardiomyocytes from guinea pig or rabbit |
|
Measured parameters |
Whole cell current of calcium channels, mainly L-type calcium channels |
|
Test concentrations |
4 doses for IC50 |
|
Data point repeats |
N ≥ 3 for each concentration |
Note: 1) Positive control: 0.03 mM CdCl2 is used in the assay to ensure cell quality and normal response of calcium channels.
2) 1 or 2 doses could be tested if IC50 is not required.
Effect of inhibitor (CdCl2) on the L-type Ca channel in cardiomyocytes isolated from guinea pig
