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In vitro cellular level

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Primary cardiac myocytes in guinea pig or rabbit- Ca channel

Time2020-04-20 15:13:14 Hits230

In vitro electrophysiology ICa assay in primary cardiomyocytes:

Assay platform/method

Conventional patch clamp

Cells used in the assay

Acutely isolated cardiomyocytes from guinea pig or rabbit

Measured parameters

Whole cell current of calcium channels, mainly L-type calcium channels

Test concentrations

4 doses for IC50

Data point repeats

N ≥ 3 for each concentration

Note: 1) Positive control: 0.03 mM CdCl2 is used in the assay to ensure cell quality and normal response of calcium channels.

2) 1 or 2 doses could be tested if IC50 is not required.

Effect of inhibitor (CdCl2) on the L-type Ca channel in cardiomyocytes isolated from guinea pig

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