Kv1.5

Kv1.5 belongs to delayed rectifying voltage-gated potassium channel family, it plays a critical role in restoring the resting cell membrane potential after depolarization. Mutations of Kv1.5 gene are related to atrial fibrillation and sudden death. Kv1.5 is also involved in the regulation of insulin secretion and pulmonary vascular constriction. In human heart, Kv1.5 mediates the ultra rapid delayed rectifier current Ikur which contributes to cellular repolarization and cardiac action potential phase 1 and phase 2. Kv1.5 is selectively expressed in atrial myocytes, thus significantly important in regulating atrial action potential duration (APD).

PharmaCore Labs provides manual patch clamp human Kv1.5 assay to evaluate drug's effect on Kv1.5 channel. This assay could serve as primary or secondary in vitro screening method for drug discovery targeting Kv1.5 as well as one of the tests in the preclinical cardiac safety evaluation panel.

In vitro electrophysiology Kv1.5 assay with manual patch clamp:

Note: 1) Positive control: 5 mM 4-AP is used in the assay to ensure cell quality and normal response.

2) 1 or 2 doses could be tested if IC50 or EC50 is not required.

Inhibition effect of 4-AP on Kv1.5 Potassium ion channel