Main Office, PCL
Address: Haimen Biotech and Pharmaceutical Park, Riverside New District, Haimen, Jiangsu Province, China
Tel：+86 0513 68015200
Email:infor@pharmacorelab.com
Website: www.pharmacorelab.com
Branch Office, PCL
Address: Block 6D, 780 Cailun Road, China (Shanghai) pilot free trade zone. infor@pharmacorelab.com
hERG channel is critical in the repolarization stage of the heart. Congenital mutation or drug inhibition of hERG channel could prolong QT interval in ECG, which potentially cause TdP (Torsades de pointes), a fatal type of polymorphic ventricular tachycardia in severe cases. On the other hand, significant drug potentiation of hERG channel could shorten QT interval, leading to cardiac arrhythmia as well.
PharmaCore Labs provides manual patch clamp functional hERG assay to evaluate drug's inhibition or potentiation effect on hERG channel.
In vitro electrophysiology functional hERG assay with manual patch clamp:
|
Assay platform/method |
Conventional patch clamp |
|
Cells used in the assay |
HEK293 hERG stable cell line |
|
Measured parameters |
Whole cell tail current of hERG channels |
|
Test concentrations |
5 doses for IC50 |
|
Data point repeats |
N ≥ 3 for each concentration |
Note: 1) Positive control: 30 μM Quinidine is used in the assay to ensure cell quality and normal response.
2) 1 or 2 doses could be tested if IC50 is not required.
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Inhibition effect of different concentrations of quinidine on hERG tail currents
The stability curve of hERG IC50 of quinidine over years
